Immunohistochemical Characterization of Tumor-Associated Macrophages in Canine Lymphomas

Macrophages have been confirmed to play a significant role in the behavior of human lymphomas, albeit no consistent data are so far available canine lymphomas. The present study characterizes macrophages cases nodal lymphoma and their relationship with histological grade immunophenotype. Samples from lymph nodes 25 dogs diagnosed were selected. Immunohistochemistry was used determine tumor immunophenotype (CD3 CD20 antibodies) macrophage characterization (Iba1, MAC387, CD204, CD163 iNOS antibodies). Macrophage counting performed 10 randomly selected, high-power fields per sample. Generalized linear models Poisson distribution for statistical analysis. A significantly greater number (Iba1+) detected high-grade B-cell highest amount both M1 (iNOS+) M2 (CD204+ CD163+) subtypes observed High-grade lymphomas showed CD204+ CD163+ cells recently recruited MAC387+ macrophages. latter most abundant T than In conclusion, population is which constitute heterogeneous that shows variations immunohistochemical profile according